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pethis6tevlic cloning vector 1b  (Addgene inc)


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    Structured Review

    Addgene inc pethis6tevlic cloning vector 1b
    Pethis6tevlic Cloning Vector 1b, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 119 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet+his6+tev+lic+cloning+vector+1b/pET+His6+TEV+LIC+cloning+vector+(1B)+(Plasmid+%2329653)/pmc13044922-77-1-11
    Average 94 stars, based on 119 article reviews
    pethis6tevlic cloning vector 1b - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Cloning:

    Article Title: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery
    Article Snippet: .. [ ] The pET vectors are based on pET His6 TEV LIC cloning vector (1B) from Scott Gradia (Addgene plasmid # 29 653, Addgene, Cambridge, MA, USA). ..

    Article Title: Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication
    Article Snippet: .. pET His6 TEV LIC cloning vector (1B) , Addgene , Plasmid #29653. .. DNA fragment Annexin A4 T7D , GeneArt Thermo Fisher Scientific , see .

    Article Title: The intracellular journey of DNA delivered with the protein-based transfection system TFAMoplex and Lipofectamine.
    Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Scott Gradia (Addgene plasmid #29653, Addgene, Cambridge, MA, USA). .. For the expression of TFAM-VRK1, and TFAM-VRK1-bZIP, the corresponding plasmids were transformed into chemically competent E. coli BL21(DE3)pLysS cells (L1195, Promega AG, Dübendorf, Switzerland), followed by cultivation in 700 mL LB medium containing 50 mg/L kanamycin and 0.2 % glucose.

    Article Title: VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection
    Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Scott Gradia (Addgene plasmid #29653, Addgene, Cambridge, MA, USA). .. For the TFAM fusion constructs and SpyCatcher-VRK1, and SpyCatcher-dVRK1, the respective plasmids were transformed into chemically competent E. coli BL21(DE3)pLysS (L1195, Promega AG, Dübendorf, Switzerland).

    Article Title: From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes.
    Article Snippet: The gene encoding the full-length putative M23/37 peptidase from S. thermophilus NCTC10353 was codon-optimized and synthesized by BioCat Company (Germany). .. A C-terminal fragment containing the enzymatically active domain (EAD) StM23 (residues 97–222 of VDG62699.1) was subcloned into the pET His6 TEV LIC cloning vector (1B), a plasmid provided by Scott Gradia (Addgene plasmid #29653), following the protocol provided by Addgene. ..

    Article Title: From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes
    Article Snippet: The gene encoding the full-length putative M23/37 peptidase from S. thermophilus NCTC10353 was codon-optimized and synthesized by BioCat Company (Germany). .. A C-terminal fragment containing the enzymatically active domain (EAD) StM23 (residues 97–222 of VDG62699.1 ) was subcloned into the pET His6 TEV LIC cloning vector (1B), a plasmid provided by Scott Gradia (Addgene plasmid #29653), following the protocol provided by Addgene. ..

    Plasmid Preparation:

    Article Title: Modular Calcium‐Responsive and CD9‐Targeted Phospholipase System Enhancing Endosomal Escape for DNA Delivery
    Article Snippet: .. [ ] The pET vectors are based on pET His6 TEV LIC cloning vector (1B) from Scott Gradia (Addgene plasmid # 29 653, Addgene, Cambridge, MA, USA). ..

    Article Title: Encapsulation of protein/DNA complexes into unilamellar liposomes via annexin-mediated membrane recruitment and sonication
    Article Snippet: .. pET His6 TEV LIC cloning vector (1B) , Addgene , Plasmid #29653. .. DNA fragment Annexin A4 T7D , GeneArt Thermo Fisher Scientific , see .

    Article Title: The intracellular journey of DNA delivered with the protein-based transfection system TFAMoplex and Lipofectamine.
    Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Scott Gradia (Addgene plasmid #29653, Addgene, Cambridge, MA, USA). .. For the expression of TFAM-VRK1, and TFAM-VRK1-bZIP, the corresponding plasmids were transformed into chemically competent E. coli BL21(DE3)pLysS cells (L1195, Promega AG, Dübendorf, Switzerland), followed by cultivation in 700 mL LB medium containing 50 mg/L kanamycin and 0.2 % glucose.

    Article Title: VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection
    Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Scott Gradia (Addgene plasmid #29653, Addgene, Cambridge, MA, USA). .. For the TFAM fusion constructs and SpyCatcher-VRK1, and SpyCatcher-dVRK1, the respective plasmids were transformed into chemically competent E. coli BL21(DE3)pLysS (L1195, Promega AG, Dübendorf, Switzerland).

    Article Title: From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes.
    Article Snippet: The gene encoding the full-length putative M23/37 peptidase from S. thermophilus NCTC10353 was codon-optimized and synthesized by BioCat Company (Germany). .. A C-terminal fragment containing the enzymatically active domain (EAD) StM23 (residues 97–222 of VDG62699.1) was subcloned into the pET His6 TEV LIC cloning vector (1B), a plasmid provided by Scott Gradia (Addgene plasmid #29653), following the protocol provided by Addgene. ..

    Article Title: From discovery to potential application: engineering a novel M23 peptidase to combat Listeria monocytogenes
    Article Snippet: The gene encoding the full-length putative M23/37 peptidase from S. thermophilus NCTC10353 was codon-optimized and synthesized by BioCat Company (Germany). .. A C-terminal fragment containing the enzymatically active domain (EAD) StM23 (residues 97–222 of VDG62699.1 ) was subcloned into the pET His6 TEV LIC cloning vector (1B), a plasmid provided by Scott Gradia (Addgene plasmid #29653), following the protocol provided by Addgene. ..

    other:

    Article Title: VWA7 - a putative human phosphatidylcholine-specific phospholipase CA.
    Article Snippet: Synthesized gene fragments were purchased from Twist Biosciences (South San Francisco, CA, USA).

    Article Title: Rapid selection of transgenic mammalian cells via diphtheria toxin resistance.
    Article Snippet: CMV-mEGFP-CREB was a gift from Ryohei Yasuda (Addgene plasmid 137001).

    Derivative Assay:

    Article Title: The intracellular journey of DNA delivered with the protein-based transfection system TFAMoplex and Lipofectamine.
    Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Scott Gradia (Addgene plasmid #29653, Addgene, Cambridge, MA, USA). .. For the expression of TFAM-VRK1, and TFAM-VRK1-bZIP, the corresponding plasmids were transformed into chemically competent E. coli BL21(DE3)pLysS cells (L1195, Promega AG, Dübendorf, Switzerland), followed by cultivation in 700 mL LB medium containing 50 mg/L kanamycin and 0.2 % glucose.

    Article Title: VRK1 co-delivery mitigates DNA clustering by BAF in TFAMoplex transfection
    Article Snippet: .. All pET vectors used in this study are derived from the pET His6 TEV LIC cloning vector (1B), developed by Scott Gradia (Addgene plasmid #29653, Addgene, Cambridge, MA, USA). .. For the TFAM fusion constructs and SpyCatcher-VRK1, and SpyCatcher-dVRK1, the respective plasmids were transformed into chemically competent E. coli BL21(DE3)pLysS (L1195, Promega AG, Dübendorf, Switzerland).



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